By: Daniel Carr and Brian Wasko
The Kaeberlein Lab yeast replicative life span team consists of more than twenty researchers, most of whom are undergraduates at the University of Washington, working in shifts on 5 yeast dissecting microscopes. We perform microdissection of daughter cells away from dividing mother cells in order to quantify the number of daughter cells a yeast mother cell will produce (the cell’s replicative life span). This assay can assess the effect of genotype, as well as dietary restriction, drug intervention, and other environmental factors, on lifespan. This is a moderate-throughput method where various experiments from multiple projects in the lab, as well as collaborative experiments with other groups, are performed. We quantify replicative life span for approximately thousands of cells every week.